Combination of diffuse reflectance and fluorescence imaging of turbid media

Jianan Y. Qu; Jianwen Hua; Zhijian Huang

doi:10.1117/12.382749

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19 April 2000 Combination of diffuse reflectance and fluorescence imaging of turbid media

Jianan Y. Qu, Jianwen Hua, Zhijian Huang

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Proceedings Volume 3917, Optical Biopsy III; (2000) https://doi.org/10.1117/12.382749
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

We have used the cross-polarization method to eliminate the specular reflection and enhance the diffusive back- scattering of polarized fluorescence excitation light from the turbid media. The image of the ratio between fluorescence and cross-polarized reflection provides a map of the fluorescence yield, define das the ratio of the autofluorescence emission to the illumination incidence, over the surface of the turbid media when excitation and collection of fluorescence are highly inhomogeneous. The simple ratio imaging technique shows the feasibility to detect early cancer, which usually starts from the superficial layer of tissue, based on the contrast in the fluorescence yield between lesion and normal tissue.

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Jianan Y. Qu, Jianwen Hua, and Zhijian Huang "Combination of diffuse reflectance and fluorescence imaging of turbid media", Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); https://doi.org/10.1117/12.382749

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