DNA and protein change in tissues probed by Kubelka-Munk spectral function

Yuanlong Yang; Edward J. Celmer M.D.; Jason A. Koutcher M.D.; Robert R. Alfano

doi:10.1117/12.382744

19 April 2000 DNA and protein change in tissues probed by Kubelka-Munk spectral function

Yuanlong Yang, Edward J. Celmer M.D., Jason A. Koutcher M.D., Robert R. Alfano

Proceedings Volume 3917, Optical Biopsy III; (2000) https://doi.org/10.1117/12.382744
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Normal, fibroadenoma, malignant, and adipose breast tissues were investigated using Kubelka-Munk Spectral Function (KMSF). The spectral features in KMSF were identified and compared with absorption spectra determined by transmission measurements. A specified spectral feature measured in adipose tissue was assigned to (beta) -carotene, which can be used to separate fat form other molecular components in breast tissues. The peaks of (KMF) at 260nm and 280nm were attributed to DNA and proteins. The signal amplitude over 255nm to 265nm and 275nm to 285nm were found to be different for malignant fibroadenoma, and normal tissues.

Citation Download Citation

Yuanlong Yang, Edward J. Celmer M.D., Jason A. Koutcher M.D., and Robert R. Alfano "DNA and protein change in tissues probed by Kubelka-Munk spectral function", Proc. SPIE 3917, Optical Biopsy III, (19 April 2000); https://doi.org/10.1117/12.382744

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