Rapid quantification of the clinically important analytes in sub-ul simulating human sera

Jianan Y. Qu; Lan Shao

doi:10.1117/12.384938

8 May 2000 Rapid quantification of the clinically important analytes in sub-μl simulating human sera

Jianan Y. Qu, Lan Shao

Proceedings Volume 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques; (2000) https://doi.org/10.1117/12.384938
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Near IR Raman spectroscopy has been successfully used to analyze major metabolites in simulating human serum samples quantitatively. We demonstrated that the optical waveguide based Raman cell and signal collector can significantly improve the collection efficiency of inherently weak Raman signal. The acquisition time for the Raman signal with decent SNR has been reduced to 10 seconds. The sample container, a quartz capillary with length of 20 mm and diameter of 400 micrometers , is easy to produce and disposable. The Raman signal was normalized to the dominant water peak at 3350 cm^-1. This self-calibration method makes the measurement insensitive to the fluctuation of the excitation and misalignment of the collection system. A partial least squares method was used to predict the analyte concentrations of interest in the simulating human sera. The prediction accuracy of albumin, globulin, triactin, urea and glucose is greatly acceptable for clinical diagnostics. The results of this research encourage the development of practical Raman system for clinical diagnostics.

Citation Download Citation

Jianan Y. Qu and Lan Shao "Rapid quantification of the clinically important analytes in sub-μl simulating human sera", Proc. SPIE 3918, Biomedical Spectroscopy: Vibrational Spectroscopy and Other Novel Techniques, (8 May 2000); https://doi.org/10.1117/12.384938

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