2 May 2000 Confocal imaging through scattering media with a volume holographic filter
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Proceedings Volume 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII; (2000) https://doi.org/10.1117/12.384180
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
The use of a volume holographic filter as collector element in a confocal system imaging through scattering (turbid) material is described. We show that the penetration depth of the volume holographic system is de-coupled from the scatter noise discrimination properties, and is potentially more advantageous than the traditional confocal microscope. Since the volume-holographic filter is a matched-filter, the penetration depth is dependent on the mismatch of the refractive index of the sample being imaged relative to the recording conditions. We present a method to overcome this limitation based on using a pre-compensating index matching film during the recording of the volume hologram. An improvement of the penetration depth is shown experimentally.
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Michal Balberg, Michal Balberg, George Barbastathis, George Barbastathis, Sergio Fantini, Sergio Fantini, David J. Brady, David J. Brady, } "Confocal imaging through scattering media with a volume holographic filter", Proc. SPIE 3919, Three-Dimensional and Multidimensional Microscopy: Image Acquisition Processing VII, (2 May 2000); doi: 10.1117/12.384180; https://doi.org/10.1117/12.384180
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