27 April 2000 External optical imaging of freely moving mice with green fluorescent protein-expressing metastatic tumors
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Proceedings Volume 3921, Optical Diagnostics of Living Cells III; (2000) https://doi.org/10.1117/12.384220
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
We report here a new approach to genetically engineering tumors to become fluorescence such that they can be imaged externally in freely-moving animals. We describe here external high-resolution real-time fluorescent optical imaging of metastatic tumors in live mice. Stable high-level green flourescent protein (GFP)-expressing human and rodent cell lines enable tumors and metastasis is formed from them to be externally imaged from freely-moving mice. Real-time tumor and metastatic growth were quantitated from whole-body real-time imaging in GFP-expressing melanoma and colon carcinoma models. This GFP optical imaging system is highly appropriate for high throughput in vivo drug screening.
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Meng Yang, Meng Yang, Eugene Baranov, Eugene Baranov, Hiroshi Shimada, Hiroshi Shimada, A. R. Moossa, A. R. Moossa, Robert M. Hoffman, Robert M. Hoffman, } "External optical imaging of freely moving mice with green fluorescent protein-expressing metastatic tumors", Proc. SPIE 3921, Optical Diagnostics of Living Cells III, (27 April 2000); doi: 10.1117/12.384220; https://doi.org/10.1117/12.384220
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