21 April 2000 Single molecule detection in single living cells
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Proceedings Volume 3922, Scanning and Force Microscopies for Biomedical Applications II; (2000) https://doi.org/10.1117/12.383337
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
The ability to detect a single analyte molecule represents the ultimate in sensitivity. Single molecule detection has emerged as a powerful tool to characterize heterogeneous systems, in which traditional bulk sampling methods provide a signal averaged over a large number of analytes. Traditionally, single molecule measurements have required highly controlled experimental conditions using ultrapure solvents to create a minimum level of interference. These constraints have primarily limited this technique to examination of systems in vitro. In this report we present the first instance of real-time single molecule detection in living cells. Our experimental approach allows dynamic monitoring of individual fluorophores in vivo, despite the highly complex cellular environment.
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Tyler A. Byassee, Tyler A. Byassee, Warren C. W. Chan, Warren C. W. Chan, Shuming Nie, Shuming Nie, } "Single molecule detection in single living cells", Proc. SPIE 3922, Scanning and Force Microscopies for Biomedical Applications II, (21 April 2000); doi: 10.1117/12.383337; https://doi.org/10.1117/12.383337

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