Manipulation of lipid bilayer membranes in solution using laser tweezers and microsphere handles

Christine D. Keating; Terrence G. D'Onofrio; Anat Hatzor; Amy S. Whelpley; Michael J. Natan; Paul S. Weiss

doi:10.1117/12.384249

26 April 2000 Manipulation of lipid bilayer membranes in solution using laser tweezers and microsphere handles

Christine D. Keating, Terrence G. D'Onofrio, Anat Hatzor, Amy S. Whelpley, Michael J. Natan, Paul S. Weiss

Proceedings Volume 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation; (2000) https://doi.org/10.1117/12.384249
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The local structure of biological membranes is critically important to membrane function. Regions of very high positive and negative curvature are found in the membranes of many cells, and rapid changes in membrane curvature are integral parts of many cell activities (e.g. endo/exocytosis, cell crawling, cell division). Our goal is to understand the effects of changes in local membrane structure on membrane properties. Optical tweezers are used to control the local structure of the lipid bilayer by controlling the curvature of the membrane. We use giant (`cell-sized'), thin-walled vesicles as our membrane models. Optically trapped latex microspheres are used to deform the liposome bilayer, forming large areas of altered membrane curvature. In contrast to literature reports in which 514.5 nm light was used in optical trapping, we have not observed adhesion of uncoated latex microspheres to liposome vesicles, nor have we observed signs of rapidly increased osmotic pressure within irradiated vesicles. This indicates that the longer wavelength used in our studies (647.1 nm) is less damaging to biological membranes. Furthermore, optical trapping of vesicles with coexisting gel and fluid phase lipids did not lead to gross changes in domain structure, which would be expected upon laser-induced heating of the membrane.

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Christine D. Keating, Terrence G. D'Onofrio, Anat Hatzor, Amy S. Whelpley, Michael J. Natan, and Paul S. Weiss "Manipulation of lipid bilayer membranes in solution using laser tweezers and microsphere handles", Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); https://doi.org/10.1117/12.384249

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KEYWORDS

Optical tweezers

Latex

Luminescence

Microfluidics

Microscopes

Proteins

Digital image correlation

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