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26 April 2000 One-step conjugation of biomolecules to luminescent nanocrystals
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Proceedings Volume 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation; (2000) https://doi.org/10.1117/12.384243
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
The development of optical pulses is crucial to the elucidation of complex biological and chemical problems. Existing fluorescent labels tend to photobleach, have low luminescence, and area sensitive to the environment. These problems can be overcome by the use of semiconductor quantum dots. These ZnS-capped CdSe quantum dots are synthesized in an organic media. To use them for biological labeling, the quantum dots are made water-soluble by adsorbing bifunctional molecules onto the surface. This step is crucial for the successful conjugation of biomolecules onto the surface. In this report, a new method for the direct adsorption of biomolecules onto the surface of quantum dots is demonstrated. Biomolecules such as glutathione, mercaptusuccic acid, and histidine are directly conjugated to luminescent quantum dots by S-Zn or N-Zn bonding.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Warren C. W. Chan, Tara L. Prendergast, Manas Jain, and Shuming Nie "One-step conjugation of biomolecules to luminescent nanocrystals", Proc. SPIE 3924, Molecular Imaging: Reporters, Dyes, Markers, and Instrumentation, (26 April 2000); https://doi.org/10.1117/12.384243
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