Fluorescent labeling of DNA for genetic analysis

Steve Menchen; Scott Benson; Krishna Upadhya; Pete Theisen; Joan Hauser; Paul Kenney

doi:10.1117/12.380498

22 March 2000 Fluorescent labeling of DNA for genetic analysis

Steve Menchen, Scott Benson, Krishna Upadhya, Pete Theisen, Joan Hauser, Paul Kenney

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380498
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

Labeling of DNA with fluorescent reporter is generally accomplished by attaching fluorescent dyes to the 5'-end of primers for PCR based applications, including geno-typing and linkage mapping. Requirements of spectral resolution and chemical stability have guided us toward asymmetric fluorescein dye structures whose chemical and physical properties have been fine-tuned through substituent modification. We have developed dyes that are amenable to 5'-prime labeling on solid support as dye-amidites facilitating automated dye-primer synthesis.

Citation Download Citation

Steve Menchen, Scott Benson, Krishna Upadhya, Pete Theisen, Joan Hauser, and Paul Kenney "Fluorescent labeling of DNA for genetic analysis", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380498

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available