22 March 2000 Fluorescent labeling of DNA for genetic analysis
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Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000); doi: 10.1117/12.380498
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
Labeling of DNA with fluorescent reporter is generally accomplished by attaching fluorescent dyes to the 5'-end of primers for PCR based applications, including geno-typing and linkage mapping. Requirements of spectral resolution and chemical stability have guided us toward asymmetric fluorescein dye structures whose chemical and physical properties have been fine-tuned through substituent modification. We have developed dyes that are amenable to 5'-prime labeling on solid support as dye-amidites facilitating automated dye-primer synthesis.
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Steve Menchen, Scott Benson, Krishna Upadhya, Pete Theisen, Joan Hauser, Paul Kenney, "Fluorescent labeling of DNA for genetic analysis", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380498; https://doi.org/10.1117/12.380498
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KEYWORDS
Optical filters

Condition numbers

Spectral resolution

Genetics

Solids

Chlorine

Field emission displays

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