New spectrophotometer for easy absorbance measurement

Takeshi Taguchi; Mitsuo Hiramatsu

doi:10.1117/12.380513

22 March 2000 New spectrophotometer for easy absorbance measurement

Takeshi Taguchi, Mitsuo Hiramatsu

Author Affiliations +

Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000) https://doi.org/10.1117/12.380513
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States

Abstract

The spectrophotometer we have developed enables us to measure absorbance in a simple way without recovering and contaminating the analyte. The absorbance of the analyte can be measured with the sample solution being retained in the tip of the pipette. The traditional cuvette is totally unnecessary. Both the tip and pipette used in this spectrophotometer are commercially available. We have measured the absorbance of analytes containing DNA dissolved in water and compared them with results obtained by the conventional spectrophotometer. Both absorbance values sufficiently agree in repeated tests. The paper will present the features of our instrument and detailed results.

Citation Download Citation

Takeshi Taguchi and Mitsuo Hiramatsu "New spectrophotometer for easy absorbance measurement", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); https://doi.org/10.1117/12.380513

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