22 March 2000 Reversed enzyme activity DNA interrogation test: a new method for mutation detection
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Proceedings Volume 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing; (2000); doi: 10.1117/12.380502
Event: BiOS 2000 The International Symposium on Biomedical Optics, 2000, San Jose, CA, United States
Abstract
The Reversed Enzyme Activity DNA Interrogation Test (READITTM) System provides a new, rapid method for genotype determination. The system can be used for the analysis of single nucleotide polymorphisms as well as insertions, deletions and chromosomal rearrangements. The READITTM System is based upon driving various enzymatic reactions in the reverse direction from the direction they are normally considered. The method is based on a pyrophosphorylation reaction that is the reverse of DNA polymerization. Interrogation probes annealed to DNA fragments with which they have a 3- mismatch are not a substrate for the reaction and do not generate significant signals compared with probes that match the interrogation sequence perfectly. By interrogation of specific DNA sequences with carefully designed probes, mutant genes can be identified with speed and precision.
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Michelle Mandrekar, Ken Lewis, Donna Leippe, Ryan Olson, John W. Shultz, "Reversed enzyme activity DNA interrogation test: a new method for mutation detection", Proc. SPIE 3926, Advances in Nucleic Acid and Protein Analyses, Manipulation, and Sequencing, (22 March 2000); doi: 10.1117/12.380502; https://doi.org/10.1117/12.380502
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KEYWORDS
Acquisition tracking and pointing

Polymers

DNA profiling

Signal generators

Polymerization

Genetics

Annealing

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