14 April 2000 Confocal imaging of biological tissues using second harmonic generation
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Proceedings Volume 3927, Optical Pulse and Beam Propagation II; (2000) https://doi.org/10.1117/12.382051
Event: Symposium on High-Power Lasers and Applications, 2000, San Jose, CA, United States
A confocal microscopy imaging system was devised to selectively detect second harmonic signals generated by biological tissues. Several types of biological tissues were examined using this imaging system, including human teeth, bovine blood vessels, and chicken skin. All these tissues generated strong second harmonic signals. There is considerable evidence that the source of these signals in tissue is collagen. Collagen, the predominant component of most tissues, is known to have second order nonlinear susceptibility. This technique may have diagnostic usefulness in pathophysiological conditions characterized by changes in collagen structure including malignant transformation of nevi, progression of diabetic complications, and abnormalities in wound healing.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Beop-Min Kim, Beop-Min Kim, Patrick Christian Stoller, Patrick Christian Stoller, Karen M. Reiser, Karen M. Reiser, Juergen P. Eichler, Juergen P. Eichler, Ming Yan, Ming Yan, Alexander M. Rubenchik, Alexander M. Rubenchik, Luiz Barroca Da Silva, Luiz Barroca Da Silva, } "Confocal imaging of biological tissues using second harmonic generation", Proc. SPIE 3927, Optical Pulse and Beam Propagation II, (14 April 2000); doi: 10.1117/12.382051; https://doi.org/10.1117/12.382051

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