Immunosensing of trinitrotoluene using sol-gel glasses

Esther H. Lan; Bruce S. Dunn; Jeffrey I. Zink

doi:10.1117/12.384335

2 May 2000 Immunosensing of trinitrotoluene using sol-gel glasses

Esther H. Lan, Bruce S. Dunn, Jeffrey I. Zink

Proceedings Volume 3943, Sol-Gel Optics V; (2000) https://doi.org/10.1117/12.384335
Event: Symposium on Integrated Optoelectronics, 2000, San Jose, CA, United States

Abstract

Research in recent years has demonstrated that biological molecules such as enzymes, immunoglobulins, and other proteins can be immobilized in sol-gel derived matrices and retain their biological function. Development of glasses with encapsulated biomolecules has opened the possibility of solid-state optical biosensors for the detection and measurement of desired analytes. In this work, we report the successful use of sol-gel silica glasses with encapsulated antibodies for the detection of trinitrotoluene (TNT). Results show that both competitive immunoassay and displacement immunoassay are feasible using antibody-doped glasses, and these materials can detect TNT at < 1 ppm. Moreover, the sol-gel immobilized antibodies retained the ability to discriminate between TNT and an analog, trinitrobenzene. Finally, enhanced stability was observed in the sol-gel immobilized as compared to surface immobilized antibodies.

Citation Download Citation

Esther H. Lan, Bruce S. Dunn, and Jeffrey I. Zink "Immunosensing of trinitrotoluene using sol-gel glasses", Proc. SPIE 3943, Sol-Gel Optics V, (2 May 2000); https://doi.org/10.1117/12.384335

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