6 June 2000 Automatic registration and segmentation algorithm for multiple electrophoresis images
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We present an algorithm for registering, segmenting and quantifying multiple scanned electrophoresis images. (2D gel) Electrophoresis is a technique for separating proteins or other macromolecules in organic material according to net charge and molecular mass and results in scanned grayscale images with dark spots against a light background marking the presence of such macromolecules. The algorithm begins by registering each of the images using a non-rigid registration algorithm. The registered images are then jointly segmented using a Markov random field approach to obtain a single segmentation. By using multiple images, the effect of noise is greatly reduced. We demonstrate the algorithm on several sets of real data.
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Matthew S. Baker, Matthew S. Baker, Harald Busse, Harald Busse, Martin Vogt, Martin Vogt, "Automatic registration and segmentation algorithm for multiple electrophoresis images", Proc. SPIE 3979, Medical Imaging 2000: Image Processing, (6 June 2000); doi: 10.1117/12.387705; https://doi.org/10.1117/12.387705

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