Quantitative Analysis Of Transmitted Images

James H. Steele Jr.

doi:10.1117/12.953818

1 March 1974 Quantitative Analysis Of Transmitted Images

James H. Steele Jr.

Proceedings Volume 0040, Quantitative Imagery in the Biomedical Sciences II; (1974) https://doi.org/10.1117/12.953818
Event: Annual Technical Symposium, 1973, San Diego, United States

Abstract

Quantitative morphological analysis of the structure of biological materials or systems is generally carried out from observations made upon thin histological sections. The stereological problem involved in this type of analysis is the statistical relationship between measurements made on transmitted images and the morphology of the structure. The section is thus a statistical sample of the structure from which transmitted images are formed using optical or electron micros-copy, radiography, autoradiography, or other techniques. These images contain projections of the structural features cut by or contained within the thin sample, and thus are significantly different from reflected light images which can usually be assumed to approximate true two-dimensional samples of the structure. The stereological problems associated with the quantitative analysis of morphology from transmitted images will be considered in this paper.

Citation Download Citation

James H. Steele Jr. "Quantitative Analysis Of Transmitted Images", Proc. SPIE 0040, Quantitative Imagery in the Biomedical Sciences II, (1 March 1974); https://doi.org/10.1117/12.953818

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Image analysis

Statistical analysis

Quantitative analysis

Spherical lenses

Calibration

Optical spheres

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