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6 April 2000 Changes in autofluorescence in the skin induced by hydration of the stratum corneum
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The autofluorescence of the skin is known to originate form several different fluorphores located at different locations in the skin. The autofluorescence spectrum measured at the surface of the skin however does not only reflect the presence of different fluorophores in the sin. On its way to the surface of the skin the fluorescence spectrum is strongly influenced by the optics of the tissue. Not only are several absorption dips present in the spectra, relating to known and well characterized absorbers such as oxyhemoglobin, it is also very likely that the shape of the measured autofluorescence spectrum is influenced by scattering of light. The scattering coefficients are known to change only slowly with wavelength. Scattering artifacts in fluorescence spectra are therefore much more difficult to detect than absorption artifacts.
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M. J. H. Kessels and Hericus J. C. M. Sterenborg "Changes in autofluorescence in the skin induced by hydration of the stratum corneum", Proc. SPIE 4001, Saratov Fall Meeting '99: Optical Technologies in Biophysics and Medicine, (6 April 2000);

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