Equipment for measurement of the concentration of coordinate-bound copper in milklike dispersing liquid

Boris S. Mishenko; Ludmila V. Puchkova; Evelina Zhiguleva; Tatyana Sviridova; Olga Bodrova; Roman Povalikhin; Nadejda V. Tsymbalenko; Vladimir Gaitskhoki

doi:10.1117/12.381497

6 April 2000 Equipment for measurement of the concentration of coordinate-bound copper in milklike dispersing liquid

Boris S. Mishenko, Ludmila V. Puchkova, Evelina Zhiguleva, Tatyana Sviridova, Olga Bodrova, Roman Povalikhin, Nadejda V. Tsymbalenko, Vladimir Gaitskhoki

Author Affiliations +

Proceedings Volume 4001, Saratov Fall Meeting '99: Optical Technologies in Biophysics and Medicine; (2000) https://doi.org/10.1117/12.381497
Event: Saratov Fall Meeting '99, 1999, Saratov, Russian Federation

Abstract

The content of ceruloplasmin (Cp, multicopper oxidase, CE 1 .16.3. 1) was measured in samples of breast milk during the first month of lactation using enzymatic and inimunochemical assays. The 4-fold decrease of Cp concentration in the milk during 20 days of lactation period was demonstrated. It was shown that copper is almost completely bound to Cp. Milk ['251]-Cp administered per os to six day-old rats was transferred across the gastric and intestinal mucosa and entered the bloodstream. The special calculation shown that newborns fed by baby formula (BF) receive magnitude excess of copper ions which not packing into safety molecular cover. It is possible that the artificial feeding is the factor interfering with the copper balance in newborns and causing various copper associated disease. For the solution of this problem an experimental model of the laser analyzer, permitting selectively to measure coordinate-bound copper in dispersing fluids is design.

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Boris S. Mishenko, Ludmila V. Puchkova, Evelina Zhiguleva, Tatyana Sviridova, Olga Bodrova, Roman Povalikhin, Nadejda V. Tsymbalenko, and Vladimir Gaitskhoki "Equipment for measurement of the concentration of coordinate-bound copper in milklike dispersing liquid", Proc. SPIE 4001, Saratov Fall Meeting '99: Optical Technologies in Biophysics and Medicine, (6 April 2000); https://doi.org/10.1117/12.381497

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