Polarized fluorescence of nonrigid macromolecules in solution

A. P. Blokhin; M. F. Gelin; A. V. Uvarov

doi:10.1117/12.380127

20 March 2000 Polarized fluorescence of nonrigid macromolecules in solution

A. P. Blokhin, M. F. Gelin, A. V. Uvarov

Proceedings Volume 4002, Saratov Fall Meeting '99: Laser Physics and Spectroscopy; (2000) https://doi.org/10.1117/12.380127
Event: Saratov Fall Meeting '99, 1999, Saratov, Russian Federation

Abstract

Depolarization of fluorescence of labeled macromolecules is determined by the second rank time orientational correlation functions (OCFs) of the absorption and emission dipole moments. To calculate the OCFs, macromolecules are conventionally modeled by the set of spherical particles (beads). To investigate the influence of the inertial and memory effects on the orientational relaxation, the diffusion equation with memory is used to perform illustrative calculations of the OCFs for a 2D system consisting of three beads joined by two rigid rods. These calculations reveal that under certain conditions the OCFs exhibit an oscillatory behavior, which is irreproducible within the standard diffusion equation.

Citation Download Citation

A. P. Blokhin, M. F. Gelin, and A. V. Uvarov "Polarized fluorescence of nonrigid macromolecules in solution", Proc. SPIE 4002, Saratov Fall Meeting '99: Laser Physics and Spectroscopy, (20 March 2000); https://doi.org/10.1117/12.380127

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available