20 March 2000 Polarized fluorescence of nonrigid macromolecules in solution
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Depolarization of fluorescence of labeled macromolecules is determined by the second rank time orientational correlation functions (OCFs) of the absorption and emission dipole moments. To calculate the OCFs, macromolecules are conventionally modeled by the set of spherical particles (beads). To investigate the influence of the inertial and memory effects on the orientational relaxation, the diffusion equation with memory is used to perform illustrative calculations of the OCFs for a 2D system consisting of three beads joined by two rigid rods. These calculations reveal that under certain conditions the OCFs exhibit an oscillatory behavior, which is irreproducible within the standard diffusion equation.
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A. P. Blokhin, A. P. Blokhin, M. F. Gelin, M. F. Gelin, A. V. Uvarov, A. V. Uvarov, "Polarized fluorescence of nonrigid macromolecules in solution", Proc. SPIE 4002, Saratov Fall Meeting '99: Laser Physics and Spectroscopy, (20 March 2000); doi: 10.1117/12.380127; https://doi.org/10.1117/12.380127

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