28 July 2000 Investigation of labeling FRET pairs to biomolecules for the development of dual-receptor biosensors
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Abstract
We report herein on the development of a dual receptor detection method for enhanced biosensor monitoring. The proposed scheme requires the integration of a chemical transducer system with two unique protein receptors that bind to a single biological agent. Optical transduction occurs because the two protein receptors are tagged with special molecular groups. When bound to a single biological agent, these fluorescently labeled proteins undergo a change in fluorescence. This `fluorescent switching' relies on the well-known mechanisms of fluorescent resonance energy transfer (FRET). The paper focuses on the investigation and optimization of the chemical transduction system (FRET). A number of FRET dye pairs were tested in a spectrofluorimeter, and promising FRET pairs (FITC/TRITC and DMACA/FITC) were further tagged to the protein, avidin and its ligand, biotin. Due to their affinities, the FRET-tagged biomolecules combine in solution, resulting in a stable, fluorescent signal from the acceptor FRET dye with a simultaneous decrease in fluorescent signal from the donor FRET dye. The results indicate that the determined FRET pairs can be utilized in the development of dual receptor sensors.
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Sheila A. Grant, Sheila A. Grant, Juntao Xu, Juntao Xu, Edward J. Bergeron, Edward J. Bergeron, Jennifer Mroz, Jennifer Mroz, Amanda Rauschel, Amanda Rauschel, } "Investigation of labeling FRET pairs to biomolecules for the development of dual-receptor biosensors", Proc. SPIE 4036, Chemical and Biological Sensing, (28 July 2000); doi: 10.1117/12.394059; https://doi.org/10.1117/12.394059
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