Laser-induced priming of human blood leukocytes

Tatyana V. Chichuk; Eugeny Ph. Stranadko; I. A. Strashkevich; Gennady I. Klebanov

doi:10.1117/12.375254

30 December 1999 Laser-induced priming of human blood leukocytes

Tatyana V. Chichuk, Eugeny Ph. Stranadko, I. A. Strashkevich, Gennady I. Klebanov

Proceedings Volume 4059, Laser Use in Oncology II; (1999) https://doi.org/10.1117/12.375254
Event: Selected Papers on Laser Use in Oncology II, 1999, none, Armenia

Abstract

We investigated the influence of He-Ne ((lambda) equals 632.8 nm) laser irradiation (LI) on a functional activity of human blood leucocytes. The method of luminol-dependent chemiluminescence with the zymosan-activated phagocytes was used. The leucocytes were irradiated without and in the presence of autologic human blood plasma, containing of the endogenous (porphyrins) and/or exogenous (phthalocyanine) photosensitizers. The LI initiated a priming of the leucocytes. Priming revealed itself after the activation of the phagocytes by zymosan. The changes of the calcium concentration in leucocytes cytoplasm were studied too. Fluorimetric method with Fura-2AM was used for this. The laser irradiation initiated the changes of the calcium concentration in the leucocytes cytoplasm. All the investigating parameters depended on the irradiation dose and on the concentration of photosensitizers. The results of this work allowed to formulate the main theses of the free radical mechanism of the low intensive laser irradiation action on human blood leucocytes.

Citation Download Citation

Tatyana V. Chichuk, Eugeny Ph. Stranadko, I. A. Strashkevich, and Gennady I. Klebanov "Laser-induced priming of human blood leukocytes", Proc. SPIE 4059, Laser Use in Oncology II, (30 December 1999); https://doi.org/10.1117/12.375254

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