Surface intrinsic fluorescence spectroscopy of proteins using a UV linearly polarized pulsed laser beam

S. Yapoudjian; M. Ivanova; Olivier P. Uteza; Vladimir I. Marine; Marc L. Sentis

doi:10.1117/12.383460

24 April 2000 Surface intrinsic fluorescence spectroscopy of proteins using a UV linearly polarized pulsed laser beam

S. Yapoudjian, M. Ivanova, Olivier P. Uteza, Vladimir I. Marine, Marc L. Sentis

Proceedings Volume 4071, International Conference on Atomic and Molecular Pulsed Lasers III; (2000) https://doi.org/10.1117/12.383460
Event: International Conference on Atomic and Molecular Pulsed Lasers III, 1999, Tomsk, Russian Federation

Abstract

The proposed new interfacial spectroscopic method allows to measure the fluorescence emission spectra of the two tryptophans of the Bovine Serum Albumin (BSA) during its adsorption at the air/water and egg-lecithin (egg-PC)/water interfaces, using an UV excimer laser. Surface fluorescence spectroscopy shows changes in the spectroscopic properties of adsorbed BSA, spread BSA and mixed egg-PC/BSA films. These results are related to the surface pressure measurements which characterize the different BSA surface organizations.

Citation Download Citation

S. Yapoudjian, M. Ivanova, Olivier P. Uteza, Vladimir I. Marine, and Marc L. Sentis "Surface intrinsic fluorescence spectroscopy of proteins using a UV linearly polarized pulsed laser beam", Proc. SPIE 4071, International Conference on Atomic and Molecular Pulsed Lasers III, (24 April 2000); https://doi.org/10.1117/12.383460

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