Translator Disclaimer
4 July 2000 Applications of optical tweezers and an integrated force measurement module for biomedical research
Author Affiliations +
Abstract
Optical tweezers are useful for manipulating biological samples and measuring biological forces. In the present study, we have integrated a forward scatter analysis (FORSA) module in the single-beam gradient force optical tweezers. The entire set-up was then incorporated onto an inverted microscope. In the FORSA module an Helium-Neon probing laser was spotted (at a slightly out-of-focus way) onto the object being trapped by the infrared laser-based tweezers and generated a diffraction pattern. Imagines of the diffraction pattern were captured by a charge- coupled device (CCD), and digitized and processed by a computer. Wed demonstrated that tracking the amplified diffraction pattern war much more precise to determine the movement of the object within the trap than analyzing the minute motion of the object itself. Displacement of the object could then be translated into the force being applied by the tweezers. Also, using an algorithm developed in the lab, we were able to follow the movement of the scattering pattern at a temporal resolution close to video rate. We have used this system to investigate the binding force associate with cell-cell interactions and modular interactions. In these studies. A cell was carefully positioned to make contact with another cell or a microparticle coated with proteins of interest by optical tweezers in a well-controlled manner. During these events, we noted a progressive increase of cell adhesion at the immediate early period (i.e., a few minutes after initial contact) of cell-cell interactions. Also, binding of a disintegrin, rhodostomin, and its mutant to the counterpart integrin on the cell surface could be assessed with great convenience and accuracy. Our results demonstrated that addition of the forward scatter analysis module to convention optical tweezers provides an effective and convenient way for monitoring biological activities in situ and measuring changes of biological forces with precision.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jin-Wu Tsai, Bing-Yao Liao, Chun-Cheng Huang, Wen-Liang Hwang, Da-Wei Wang, Arthur E. T. Chiou, and Chi-Hung Lin "Applications of optical tweezers and an integrated force measurement module for biomedical research", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); https://doi.org/10.1117/12.390549
PROCEEDINGS
9 PAGES


SHARE
Advertisement
Advertisement
Back to Top