4 July 2000 Multiphoton fluorescence spectroscopy of flourescent bioprobes and biomolecules
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Abstract
Multi-photon fluorescence spectra of a number of commonly used biological probes were measured in this study. Significant spectral variation has been detected between single and multi- photon excitation. The result is important for the proper selection of spectral setting/dichroic beam splitter in the set- up of a multi-photon fluorescence microscope. The information can also be useful in the detection of multi-photon fluorescence in bio-chip technology. In addition, we have investigated a few highly fluorescent bio-molecules commonly found in plant cells.
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Ping Chin Cheng, Ping Chin Cheng, Bai-Ling Lin, Bai-Ling Lin, Fu-Jen Kao, Fu-Jen Kao, Chi-Kuang Sun, Chi-Kuang Sun, Yung-Shun Wang, Yung-Shun Wang, Tzu-Ming Liu, Tzu-Ming Liu, YiMin Wang, YiMin Wang, JianCheng Chen, JianCheng Chen, Mao-Kuo Huang, Mao-Kuo Huang, Iain Johnson, Iain Johnson, } "Multiphoton fluorescence spectroscopy of flourescent bioprobes and biomolecules", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390527; https://doi.org/10.1117/12.390527
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