4 July 2000 Multiphoton microspectroscopy of biological specimens
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The non-linear nature of multi-photon fluorescence excitation restricts the fluorescing volume to the vicinity of the focal point. As a result, the technology has the capacity for micro- spectroscopy of biological specimen at high spatial resolution. Chloroplasts in mesophyll protoplast of Arabidopsis thaliana and maize stem sections were used to demonstrate the feasibility of multi-photon fluorescence micro-spectroscopy at subcellular compartments. Time-lapse spectral recording provides a means for studying the response of cell organelles to high intensity illumination.
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Bai-Ling Lin, Bai-Ling Lin, Fu-Jen Kao, Fu-Jen Kao, Ping Chin Cheng, Ping Chin Cheng, Chi-Kuang Sun, Chi-Kuang Sun, RangWu Chen, RangWu Chen, YiMin Wang, YiMin Wang, JianCheng Chen, JianCheng Chen, Yung-Shun Wang, Yung-Shun Wang, Tzu-Ming Liu, Tzu-Ming Liu, Mao-Kuo Huang, Mao-Kuo Huang, "Multiphoton microspectroscopy of biological specimens", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390530; https://doi.org/10.1117/12.390530

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