4 July 2000 Second-harmonic generation microscopy of tooth
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Abstract
In this study, we have developed a high performance microscopic system to perform second-harmonic (SH)imaging on a tooth. The high sensitivity of the system allows an acquisition rate of 300 seconds/frame with a resolution at 512x512 pixels. The surface SH signal generated from the tooth is also carefully verified through micro-spectroscopy, polarization rotation, and wavelength tuning. In this way, we can ensure the authenticity of the signal. The enamel that encapsulates the dentine is known to possess highly ordered structures. The anisotrophy of the structure is revealed in the microscopic SH images of the tooth sample.
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Fu-Jen Kao, Fu-Jen Kao, Yung-Shun Wang, Yung-Shun Wang, Mao-Kuo Huang, Mao-Kuo Huang, Sheng-Lung Huang, Sheng-Lung Huang, Ping Chin Cheng, Ping Chin Cheng, } "Second-harmonic generation microscopy of tooth", Proc. SPIE 4082, Optical Sensing, Imaging, and Manipulation for Biological and Biomedical Applications, (4 July 2000); doi: 10.1117/12.390534; https://doi.org/10.1117/12.390534
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