15 December 2000 Characterization of biofilms with a fiber optic spectrometer
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Proceedings Volume 4087, Applications of Photonic Technology 4; (2000) https://doi.org/10.1117/12.406344
Event: 2000 International Conference on Application of Photonic Technology (ICAPT 2000), 2000, Quebec City, Canada
Abstract
Optical sensing is one promising approach to monitor bioflims in an early stage. Generally, natural bioflims are quite inhomogeneous, therefore we start the investigation with suspensions of dead bacteria in water as a simple model for a bioflim. An experimental arrangement based on a white light fiber optic spectrometer is used for measuring the density of a thin film with a local resolution in the order of several tim. The method is applied with model biofilms. In a computer controlled procedure reflectance spectra may be recorded at different positions in the x-y plane. Scanning through thin suspension regions of bacteria between glass plates allows an estimation of the refractive index of bacteria. Taking advantage of the light collecting property of the glass substrate a simple measurement of the fluorescence with local resolution is demonstrated as well.
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S. Krautwald, S. Krautwald, A. Tonyali, A. Tonyali, B. Fellerhoff, B. Fellerhoff, Hilmar Franke, Hilmar Franke, A. Tamachkiarov, A. Tamachkiarov, T. Griebe, T. Griebe, H. C. Flemming, H. C. Flemming, } "Characterization of biofilms with a fiber optic spectrometer", Proc. SPIE 4087, Applications of Photonic Technology 4, (15 December 2000); doi: 10.1117/12.406344; https://doi.org/10.1117/12.406344
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