2 October 2000 Novel integrated optical sensor for DNA arrays detection
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Abstract
A novel integrated optical biosensor, that combines optical planar waveguides for illumination with microlens arrays for collection of fluorescence, is presented in this paper. This sensor is based on laser induced fluorescence detection and will be applied for biochemical or chemical applications such as fluorescence labeled DNA affinity assay. An evanescent field is generated by the optical planar waveguides, which illuminate DNA arrays on the surface of the waveguide. High signal to noise ratio and sensitivity can be achieved since the evanescent field is tightly confined within the neighborhood of the surface of waveguides and laser noise will scarcely reach the detector. Micro lens arrays with large numerous aperture are combined into the sensor to collect more fluorescent lights from DNA samples induced by the evanescent field. A high sensitive CCD is used for this multi-channel system. The sensor has advantages of compactness, multi-dimensional channel detection simultaneously, high sensitivity and good compatibility with biology samples, so it is not only suitable to be used in detection of fluorescent labeled DNA arrays, but also can be used in other life science research like fluorescence immunoassays. The compact design of the sensor allows it to be integrated in a micro total analysis system ((mu) -TAS) such as a portable DNA diagnostics device.
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Rong Yang, Rong Yang, Minxian Wu, Minxian Wu, Guofan Jin, Guofan Jin, } "Novel integrated optical sensor for DNA arrays detection", Proc. SPIE 4092, Novel Optical Systems Design and Optimization III, (2 October 2000); doi: 10.1117/12.402423; https://doi.org/10.1117/12.402423
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