2 October 2000 Parallel confocal laser microscope system using smart pixel arrays
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Abstract
The temporal resolution of a confocal laser microscope, by which three- dimensional data of specimens are obtained, usually suffers the slow speed of image acquisition devices, such as CCDs, or scanning mirrors. Here we propose a confocal microscope system where parallel optoelectronic devices are employed aiming at obtaining high frame rate of three-dimensional data that provides real time analysis of dynamical properties and adaptive feedback control of the specimen. A smart pixel yields pixel-parallel data processing capability owing to its integrated optical devices and electronic processing circuits fabricated on the same chip. A vertical-cavity surface-emitting laser (VCSEL) array is introduced for the parallel probing beams by which the optical system is simplified. In addition, pixel parallel illumination control is achievable. We have performed basic experiments on a syst em composed on an 8x8x VCSEL array, a silicon photodetector array, and processing element (PE) array. Each PE contains an arithmetic logic unit (ALU), 24- bit local memory, and electrical connections to neighboring pixels, that provides fast processing versatility such as the moving object recognition. Vibrating a sample by a piezo micro stage, we have successfully obtained a dynamical property, which is a one-dimensional moment of the moving sample, on the basis of the data obtained by the experimental system over the range of conventional video frame rate.
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Makoto Naruse, Masatoshi Ishikawa, "Parallel confocal laser microscope system using smart pixel arrays", Proc. SPIE 4092, Novel Optical Systems Design and Optimization III, (2 October 2000); doi: 10.1117/12.402414; https://doi.org/10.1117/12.402414
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