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24 October 2000 Macro optical system for biological application
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Abstract
Currently, the requirement of wide field view fluorescence observation is increasing in the biological field, but the existing microscopes have difficulty in imaging lower magnification fluorescence images. It is very easy to image in higher magnification with higher resolution and brightness using existing microscopes, but it is very difficult to image in lower magnifications that are also bright. We focused on two applications in which a macro lens would be useful. The first application is DNA array reading. The second application is In Vivo imaging. Both applications require wide observation areas and bright images which are needed to lower magnification and fluorescence capability. The macro lens needs to have high numerical aperture (N.A.) And low magnification. We also believe that if the macro lens can be attached to the existing microscopes, it will be quite useful to various applications because the scientists can then observe fluorescence images from low magnification to high magnification. To fulfill these needs, we designed macro objective lenses. Listed bellow are our macro lens' unique features. 1. Specification for the macro objective lenses are 2X, N.A. 0.14 and 4X, N.A. 0.28. These N.A. is higher than the existing microscope objective lenses of equal magnifications. 2. The 4X and 2X macro lens can be attached to existing microscopes. 3. These macro lenses can be used with the video lens such as 0.5X.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Yoshihiro Kawano, Chikara Abe, Katsuyuki Abe, Kazuhiro Hayashi, Yoko Hatta, and Keisuke Tamura "Macro optical system for biological application", Proc. SPIE 4093, Current Developments in Lens Design and Optical Systems Engineering, (24 October 2000); https://doi.org/10.1117/12.405217
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