6 July 2000 Spectrographic evaluation of patch test reactions by NIR FT Raman spectroscopy
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The aim of the present study is to examine the availability of NIR FT Raman spectroscopy in the clinical evaluation of positive allergic and irritant or doubtful patch test reactions. Instrumental measurements at 42 positive patch test sites in 16 patients were compared visually and evaluated at 48 h and 72 h in order to examine the sensitivity of this method to detect the biochemical changes occurring at the sites of the patches. Raman spectra of normal control skin and skin reacting to patches on the back were obtained with an FRA 106 Raman module on a Bruker IFS 66 optics system (Bruker, Karlsruhe, Germany). The most significant changes in the spectra were detected in the region specific for water content and protein structure in both types of reactions at 48 h and 72 h, compared to normal skin.
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Natalja Skrebova, Natalja Skrebova, } "Spectrographic evaluation of patch test reactions by NIR FT Raman spectroscopy", Proc. SPIE 4129, Subsurface Sensing Technologies and Applications II, (6 July 2000); doi: 10.1117/12.390619; https://doi.org/10.1117/12.390619

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