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19 January 2001 High-speed digital video imaging system to record cardiac action potentials
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A new digital video imaging system was developed and its performance was evaluated to analyze the spiral wave dynamics during polymorphic ventricular tachycardia (PVT) with high spatio-temporal resolution (1 ms, 0.1 mm). The epicardial surface of isolated rabbit heart stained with di- 4-ANEPPS was illuminated by 72 high-power bluish-green light emitting diodes (BGLED: (lambda) 0 500 nm, 10mw). The emitted fluorescence image (256x256 pixels) passing through a long-pass filter ((lambda) c 660nm) was monitored by a high-speed digital video camera recorder (FASTCAM-Ultima- UV3, Photron) at 1125 fps. The data stored in DRAM were processed by PC for background subtraction. 2D images of excitation wave and single-pixel action potentials at target sites during PVT induced by DC shocks (S2: 10 ms, 20 V) were displayed for 4.5 s. The wave form quality is high enough to observe phase 0 upstroke and to identify repolarization timing. Membrane potentials at the center of spiral were characterized by double-peak or oscillatory depolarization. Singular points during PVT were obtained from isophase mapping. Our new digital video-BGLED system has an advantage over previous ones for more accurate and longer time action potential analysis during spiral wave reentry.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Akira Mishima, Tatsuhiko Arafune, Ken Masamune, Ichiro Sakuma, Takeyoshi Dohi, Nitaro Shibata, Haruo Honjo, and Itsuo Kodama "High-speed digital video imaging system to record cardiac action potentials", Proc. SPIE 4158, Biomonitoring and Endoscopy Technologies, (19 January 2001);

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