Noninvasive tumor detection using spectrally-resolved in-vivo imaging

Gennady Kostenich; Sol Kimel; Zvi Malik; Arie Orenstein M.D.

doi:10.1117/12.409335

16 November 2000 Noninvasive tumor detection using spectrally-resolved in-vivo imaging

Gennady Kostenich, Sol Kimel, Zvi Malik, Arie Orenstein M.D.

Proceedings Volume 4161, Optical Biopsy and Tissue Optics; (2000) https://doi.org/10.1117/12.409335
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

A novel spectral image-analysis system was used for tumor fluorescence and reflectance imaging in an animal model and in patients. Transcutaneous fluorescence imaging was carried out on Balb/c mice bearing subcutaneous C26 colon carcinoma after intraperitoneal (i.p.) administration of 5-aminolevulinic acid (ALA), a metabolic precursor of protoporphyrin-IX (PP), and of a novel photosensitizer tetrahydroporphyrin (THP). Tumors were clearly observable by fluorescence detection using green light excitation. Tumor versus normal tissue uptake of the photosensitizing agents was determined by monitoring fluorescence intensity. Maximal PP accumulation in tumor was observed 3 h after i.p. injection of ALA, whereas THP showed selective accumulation in tumor 24 h after administration. Reflectance spectroscopy was employed to study pigmented human skin lesions (nevus, pigmented BCC and pigmented melanoma). In the near-infrared region (800-880 nm) pigmented BCC and melanoma exhibited a differently shaped reflectance spectrum compared to normal skin and nevus. Spatially and spectrally resolved imaging, in combination with mathematical algorithms (such as normalization, spectral similarity mapping and division) allowed unambiguous detection of malignancies. Optical biopsy results from a total of 51 patients showed 45 benign nevi, 3 pigmented BCC and 3 malignant melanomas, as confirmed by histology.

Citation Download Citation

Gennady Kostenich, Sol Kimel, Zvi Malik, and Arie Orenstein M.D. "Noninvasive tumor detection using spectrally-resolved in-vivo imaging", Proc. SPIE 4161, Optical Biopsy and Tissue Optics, (16 November 2000); https://doi.org/10.1117/12.409335

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