3 November 2000 Water content and water profiles in skin measured by FTIR and Raman spectroscopy
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We present non-invasive measurements of water content in stratum corneum (SC) by FTIR spectroscopy and water content profiles across the epidermis by Raman microspectroscopy. We apply band fitting to the FTIR spectra to assess changes in SC water content following hydration. While the penetration depth of ATR-FTIR is only a few micrometers, the confocal Raman microspectrometer enables successive collection of Raman spectra at a range of depths below skin surface, with an axial resolution of ca. 5micrometers . Depth resolved water concentration measurements of the epidermis obtained by Raman spectroscopy show clear changes in the water concentration profile as a result of hydration.
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Gerald W. Lucassen, Gerald W. Lucassen, Peter J. Caspers, Peter J. Caspers, Gerwin J. Puppels, Gerwin J. Puppels, } "Water content and water profiles in skin measured by FTIR and Raman spectroscopy", Proc. SPIE 4162, Controlling Tissue Optical Properties: Applications in Clinical Study, (3 November 2000); doi: 10.1117/12.405921; https://doi.org/10.1117/12.405921

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