Fluorescence labeling and microscopic imaging of colonic mucosal transformations

Vassilis Atlamazoglou; Dido M. Yova; Nikolaos Kavantzas; Spyros Loukas

doi:10.1117/12.410634

18 December 2000 Fluorescence labeling and microscopic imaging of colonic mucosal transformations

Vassilis Atlamazoglou, Dido M. Yova, Nikolaos Kavantzas, Spyros Loukas

Proceedings Volume 4164, Laser Microscopy; (2000) https://doi.org/10.1117/12.410634
Event: EOS/SPIE European Biomedical Optics Week, 2000, Amsterdam, Netherlands

Abstract

The aim of this work was to develop an efficient fluorescence microscopic technique and selective fluoroprobes for cancer diagnosis in colon tissue sections, as well as to determine the morphological components where selective dye accumulation has occurred. For this purpose a novel fluorescence probe, Rh-B - phenyl boronic acid was synthesized and examined. This derivative distinguished clearly and consistently, healthy form neoplastic human colon tissue sections. Intense accumulation was observed at the amorphous material in the lumen of neoplastic crypts, of the colonic epithelium. To gain insight into the localization pattern of this fluoroprobe and to correlate it with mucin alterations, mucicarmine and the blue-fluorescent conjugate of wheat germ agglutinin with Alexa 350 were used. Alexa 350-WGA reacted primarily with mucin secreted in the malignant crypt lumen suggesting that this materia is rich in cialic acid and N-acetylglucosaminyl residues.

Citation Download Citation

Vassilis Atlamazoglou, Dido M. Yova, Nikolaos Kavantzas, and Spyros Loukas "Fluorescence labeling and microscopic imaging of colonic mucosal transformations", Proc. SPIE 4164, Laser Microscopy, (18 December 2000); https://doi.org/10.1117/12.410634

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