18 December 2000 Single-molecule fluorescence microscopy on nucleotide excision repair complexes using GFP fusion proteins
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Abstract
Scanning Confocal Fluorescence Microscopy is used for single molecule studies on DNA-protein complexes that occur in Nucleotide Excision Repair (NER). During DNA-damage elimination by the NER-pathway, complex protein structures assemble over DNA. It is our aim to resolve the architecture of these DNA-protein complexes and to study dynamic changes that occur in these structures. For this purpose NER- complexes are partly reconstituted onto DNA-substrates using NER-proteins fused to different Green Fluorescent Protein mutants. Here we describe the recombinant production of NER- GFP fusion proteins. Characterization of GFP fluorescence is shown together with results of GFP single molecule imaging. First results with NER-GFP fusion proteins are presented as well.
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Ine Segers-Nolten, Ine Segers-Nolten, Suzanne Rademakers, Suzanne Rademakers, Wim Vermeulen, Wim Vermeulen, Aufried Lenferink, Aufried Lenferink, Cees Otto, Cees Otto, Jan Hoeijmakers, Jan Hoeijmakers, Jan Greve, Jan Greve, } "Single-molecule fluorescence microscopy on nucleotide excision repair complexes using GFP fusion proteins", Proc. SPIE 4164, Laser Microscopy, (18 December 2000); doi: 10.1117/12.410629; https://doi.org/10.1117/12.410629
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