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18 December 2000 Two-photon fluorescence microscopy of in-vivo human skin
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We present images of human skin obtained by a two-photon fluorescence microscope, which has been optimized for in- vivo imaging. Using autofluorescence as a contrast mechanism various layers of the skin, e.g., stratum corneum, viable epidermis, basal layer and upper dermis could be clearly distinguished. A comparison between two-photon fluorescence images and reflectance images shows that there is a clear difference in the information content provided by both techniques. In particular, the fluorescence images of the dermis show detail that is absent in the reflectance images.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rob F. M. Hendriks and Gerald W. Lucassen "Two-photon fluorescence microscopy of in-vivo human skin", Proc. SPIE 4164, Laser Microscopy, (18 December 2000);

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