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11 October 2000 Automatic alignment of electron tomography images using markers
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Electron tomography means reconstructing the interior of an object from its electron microscope images. In order to successfully perform the 3D reconstruction, the motion between consecutive images must be solve, i.e., the images have to be aligned or registered. Using a set of two- dimensional electron microscope images of a three- dimensional object, we propose a method where the registration is automated using small colloidal gold particles as reference markers between images. The alignment problem is divided into several subproblems: (1) Finding initial matches from successive images, (2) estimating the epipolar geometry between images, (3) finding and localizing the gold particles with subpixel accuracy in each image, (4) predicting the probably matching gold particles using the epipolar geometry with the disparity information, (5) matching and tracking the gold particles through the tilt series, and (6) optimizing the transformation parameters for the whole image set. The results show the reliability of the suggested method as well as high accuracy in registration.
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Sami Sebastian Brandt and Jukka Heikkonen "Automatic alignment of electron tomography images using markers", Proc. SPIE 4197, Intelligent Robots and Computer Vision XIX: Algorithms, Techniques, and Active Vision, (11 October 2000);

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