Registration of immunoglobuline AB/AG reaction with planar polarization interferometer

Alexei V. Nabok; Nickolaj F. Starodub; Asim K. Ray; Aseel K. Hassan

doi:10.1117/12.411710

29 December 2000 Registration of immunoglobuline AB/AG reaction with planar polarization interferometer

Alexei V. Nabok, Nickolaj F. Starodub, Asim K. Ray, Aseel K. Hassan

Proceedings Volume 4200, Biochemical and Biomolecular Sensing; (2000) https://doi.org/10.1117/12.411710
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

Immobilization of human immunoglobuline (IgG) (AG) and goat on human IGG antibodies (AB) as well as AB/AG specific reaction were studied with planar polarization interferometry (PPI). In this novel method, polarized laser beam was coupled into the planar waveguide made on silicon wafer and consisted of 20nm Si₃N₄ layer sandwiched between two 1.5 micrometers SiO₂ layers with the sensing window etched in the top SIO₂ layer. One of the immune components was deposited by means of polyelectrolyte self- assembly on top of the Si₃N₃ layer within the sensing window, P-component of the polarized light is sensitive to adsorption, while s-component serves as a reference. Thus the outcoming light intensity depends on the phase shift between s- and p-components. Different sequences of immobilization of the immune components were studied with both surface plasmon resonance (SPR) and PPI methods. It was shown that predeposition of a monolayer of protein A, which is believed to affect the orientation of the immune components, causes an additional increase in the sensitivity. PPI method allowed us to improve substantially the sensitivity towards AB/AG reaction as compared to traditional SPR method. Particularly, of specific binding of 3ng/ml AG was registered.

Citation Download Citation

Alexei V. Nabok, Nickolaj F. Starodub, Asim K. Ray, and Aseel K. Hassan "Registration of immunoglobuline AB/AG reaction with planar polarization interferometer", Proc. SPIE 4200, Biochemical and Biomolecular Sensing, (29 December 2000); https://doi.org/10.1117/12.411710

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