Control of protein absorption for MEMs and microfluidic applications on silicon surfaces using silane-based self-assembled monolayers of an oligo(ethylene oxide) derivative

Thomas W. Schneider; Laura E. Aloi; Robert C. White

doi:10.1117/12.417442

26 February 2001 Control of protein absorption for MEMs and microfluidic applications on silicon surfaces using silane-based self-assembled monolayers of an oligo(ethylene oxide) derivative

Thomas W. Schneider, Laura E. Aloi, Robert C. White

Author Affiliations +

Proceedings Volume 4205, Advanced Environmental and Chemical Sensing Technology; (2001) https://doi.org/10.1117/12.417442
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

Microfabricated devices are becoming increasingly important in biotechnology, especially in the areas of drug delivery, biological fluid analysis, and biological sensors. Interactions between fluid constituents and device surfaces become important when device dimensions reach the micrometer scale. We are reporting the synthesis and application of an octyl trichlorosilane ether of a short chain mono methylated poly(ethylene glycol) (PEG8,7) for control of protein adsorption on silicon surfaces. Self-assembled monolayers (SAMs) of the PEG8,7, a commercially available long chain poly(ethylene glycol) silane (PEG 5000), and 1H,1H,2H,2H,- perfluorooctyltrichlorosilane (13F) were prepared. These three SAM surfaces and uncoated silicon oxide were compared for control of protein adsorption of bovine serum albumin (BSA) under nonflow conditions. PEG8,7 was shown to be the best surface for inhibiting protein adsorption. The surfaces were analyzed using X-ray photoelectron spectroscopy (XPS) to quantify protein adsorption.

Citation Download Citation

Thomas W. Schneider, Laura E. Aloi, and Robert C. White "Control of protein absorption for MEMs and microfluidic applications on silicon surfaces using silane-based self-assembled monolayers of an oligo(ethylene oxide) derivative", Proc. SPIE 4205, Advanced Environmental and Chemical Sensing Technology, (26 February 2001); https://doi.org/10.1117/12.417442

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