13 March 2001 Detection and identification of animal and food pathogens using time-resolved fluorescence
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Proceedings Volume 4206, Photonic Detection and Intervention Technologies for Safe Food; (2001) https://doi.org/10.1117/12.418741
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States
Abstract
Time-reso1ved fluorescence immunoassays using dissociation enhanced lanthanide fluorescence have been developed for detection and identification of animal and food pathogens including Clostridium botulinum toxins A and B, Staphylococcus enterotoxm B (SEB), Bacillus anthracis, E. coil 0157:H7, Salmonella, Listeria and Campylobacter. Both double antibody sandwich inimunoassays and microwell filterplate assays have been developed using polyclonal, monoclonal and phage display antibodies labeled with fluorescent europium or samarium lanthanide chelates. Multiplexed dual label eiiropium and samarium assays have been performed for Salmonella simultaneously with E. coli 0157:H7, Salmonella and Listeria. Results from different laboratories are presented to demonstrate that these time-resolved fluorescent immunoassays have high sensitivity and specificity with excellent reproducibility.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Peter E. Andreotti, Peter E. Andreotti, Richard Meyer, Richard Meyer, Thomas Campbell, Thomas Campbell, Michael T. Goode, Michael T. Goode, Deborah L. Menking, Deborah L. Menking, Emily D. Myers, Emily D. Myers, Tom Palenius, Tom Palenius, Larry H. Stanker, Larry H. Stanker, } "Detection and identification of animal and food pathogens using time-resolved fluorescence", Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); doi: 10.1117/12.418741; https://doi.org/10.1117/12.418741
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