Optical biosensor for high-throughput detection of veterinary drug residues in foods

Steven R. H. Crooks; Esa Stenberg; M. Annette Johansson; Karl-Erik Hellenaes; Christopher T. Elliott

doi:10.1117/12.418721

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13 March 2001 Optical biosensor for high-throughput detection of veterinary drug residues in foods

Steven R. H. Crooks, Esa Stenberg, M. Annette Johansson, Karl-Erik Hellenaes, Christopher T. Elliott

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Proceedings Volume 4206, Photonic Detection and Intervention Technologies for Safe Food; (2001) https://doi.org/10.1117/12.418721
Event: Environmental and Industrial Sensing, 2000, Boston, MA, United States

Abstract

A new multi—channel optical biosensor instrument has been constructed for high-throughput screening analysis of drug residues in foods. The sensor uses surface plasmon resonance technology and allows either simultaneous analysis of eight samples for a single analyte or multi analyte analysis. Three muhi analyte assays kits were developed for use on the prototype instrument: clenbuterol (CBL) and ethinyl estradiol (EEO) in urine, sulfamethazine (SMT) and sulfadiazine (SDZ) in bile and SMT, SDZ and enrofloxacin (EFX) in milk. Direct analysis was performed on milk and bile samples while detection of CBL and EEO at low ppb levels required limited sample extraction. The assay/ instrument combinations were tested by end-users in laboratory and on-site environments. Sample throughput of up to 600 samples per day was achieved. Numbers of samples tested ranged from 473 samples for the urine assay to over 7500 for the bile assay. Instrument performance in the laboratory was assessed by comparison with the end-user's standard procedure. Comparison of false positive screening results and confirmed positive samples showed the results achieved by the new technique to be at least comparable with existing screening procedure with the added benefits of increased sample throughput and result generation in "real time". On site analysis showed no evidence of the technique being adversely effected by the harsher environments. The present study has clearly demonstrated that high throughput immunosensor technology can play a leading role in the detection of residues ofveterinary drugs in food.

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Steven R. H. Crooks, Esa Stenberg, M. Annette Johansson, Karl-Erik Hellenaes, and Christopher T. Elliott "Optical biosensor for high-throughput detection of veterinary drug residues in foods", Proc. SPIE 4206, Photonic Detection and Intervention Technologies for Safe Food, (13 March 2001); https://doi.org/10.1117/12.418721

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