11 October 2000 Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer
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Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000) https://doi.org/10.1117/12.403990
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China
Abstract
Green fluorescent protein based fluorescence resonance energy transfer is widely used in studies of inter-and intra-molecular dynamic interactions in alive cells. But it is a trade off between quantitative measurement and real time measurement because of the emission cross talk. In stead of using more than one excitation wavelength and several sets of filters, we proposed a more efficient correction scheme which needs only a single excitation and one filter set. This method makes use of the known emission spectra of the fluorophores and can be run in real time. The validity and advantages were demonstrated by experiments with chamaeleons.
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Tongsheng Chen, Tongsheng Chen, Shaoqun Zeng, Shaoqun Zeng, Qingming Luo, Qingming Luo, Zhihong Zhang, Zhihong Zhang, Wei Zhou, Wei Zhou, } "Data analysis in green-fluorescent-protein-based fluorescence resonance energy transfer", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000); doi: 10.1117/12.403990; https://doi.org/10.1117/12.403990
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