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11 October 2000 Two-photon fluorescence imaging of rat aorta
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Proceedings Volume 4224, Biomedical Photonics and Optoelectronic Imaging; (2000)
Event: Optics and Optoelectronic Inspection and Control: Techniques, Applications, and Instruments, 2000, Beijing, China
Two-photon excitation fluorescence microscopy has become increasingly popular in 3D fluorescence imaging of tissue samples. Observation depth is an important indicator for the performance of the technique. Using the principle of tissue optics, we analyzed quantitatively the effects of optical properties, such as absorption coefficient, scattering coefficient, and the scattering anisotropy factor of the tissue, to the observation depth. An analytical relation between the fluorescence and the observation depth has been derived and tested against experimental results for rat aorta elastic lamina.
© (2000) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xingsun Lin, Fei Sun, Hui Ma, Jingbo Zhao, Lei Jin, and Die Yan Chen "Two-photon fluorescence imaging of rat aorta", Proc. SPIE 4224, Biomedical Photonics and Optoelectronic Imaging, (11 October 2000);

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