4 May 2001 Sedimentation of immersed blood studied by OCT
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The concept of refractive index matching used for the enhancement of optical penetration depth of the whole blood is discussed on the basis of in vitro studies. It was stated that blood optical clearing is defined not only by refractive index matching effect, but also by changes of RBC size and their aggregation ability when chemicals are added. For example, for whole blood twice diluted by a saline adding of 6.5% of glycerol reduces the total attenuation coefficient from 4.2 mm-1 to 2.0 mm-1, and correspondingly increases substantially the optical penetration up to 117%. For other tested agents (three types of dextrans A, B, and C; propylene glycol; and trazograph, all at concentration 6.5%) the enhancement of penetration was from 20.5% (for low molecular dextran) up to 77.2% for propylene glycol. For non-diluted blood such effects were not very pronounced, but still glycerol at 13% has the highest enhancement up to 52% and the total attenuation coefficient was changed from 6.1 to 5.1 mm-1.
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Valery V. Tuchin, Valery V. Tuchin, Xiangqun Xu, Xiangqun Xu, Ruikang K. Wang, Ruikang K. Wang, } "Sedimentation of immersed blood studied by OCT", Proc. SPIE 4241, Saratov Fall Meeting 2000: Optical Technologies in Biophysics and Medicine II, (4 May 2001); doi: 10.1117/12.431547; https://doi.org/10.1117/12.431547


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