Measurement of intrinsic photobleaching rate by fluorescence microsampling of tissue

Brian W. Pogue; Gregory P. Fournier; Altug R. Akay; Gregory C. Burke; P. Jack Hoopes D.V.M.

doi:10.1117/12.424438

9 April 2001 Measurement of intrinsic photobleaching rate by fluorescence microsampling of tissue

Brian W. Pogue, Gregory P. Fournier, Altug R. Akay, Gregory C. Burke, P. Jack Hoopes D.V.M.

Proceedings Volume 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X; (2001) https://doi.org/10.1117/12.424438
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States

Abstract

Recently there is some interest in using photobleaching rates in vivo to correctly interpret dose deposition, and even to predict the dose deposited within tissue. There are many factors causing photobleaching rate changes related to localization, transport and oxygen availability which can confound the interpretation of a bleaching rate measured in vivo, and perhaps the most problematic issue is that the fluorescence signal can come from multiple depths within the tissue and this depth can change during the course of treatment. In this study we examine the use of a micro-fiber bundle designed to sample fluorescence from superficial depths, and use this device to illustrate that spatially localized measurements can be used to extract the intrinsic photobleaching rate of a photosensitizer within a scattering medium. At the same time, while the depth from which the tissue signal originates can be confined, in vivo measurements illustrate that multiple bleaching rates exist in vivo in addition to the spatial problem. This latter observation, suggests that bleaching in tissue is complicated enough, that spatially localized measures of intrinsic fluorescence photobleaching are needed to allow mechanistic interpretation from in vivo measurements.

Citation Download Citation

Brian W. Pogue, Gregory P. Fournier, Altug R. Akay, Gregory C. Burke, and P. Jack Hoopes D.V.M. "Measurement of intrinsic photobleaching rate by fluorescence microsampling of tissue", Proc. SPIE 4248, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy X, (9 April 2001); https://doi.org/10.1117/12.424438

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Tissue optics

Luminescence

Tissues

In vivo imaging

Scattering

Signal detection

Light scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years