27 April 2001 Effects of LLLT on malignant cells: study in vitro
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Proceedings Volume 4249, Lasers in Dentistry VII; (2001) https://doi.org/10.1117/12.424490
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
In addition to our previous report on the effects of LLLT on the proliferation of laryngeal carcinoma cells in which it was found that irradiation H.Ep.2 cells with 670nm laser results in increased cell proliferation, ti was decided to evaluate the effect of increased does of laser light. The aim of this study was to assess the effect off 635 and 670 nm laser irradiation of H.Ep.2 cells in vitro using MTT. The cells, obtained from SCC of the larynx, were routinely processed from defrost to the experimental condition. The cultures were kept either at 5 or 10 percent of FBS. Twenty- four hours after transplantation, the cells were irradiated with laser light at local light doses between 0.04 and 4.8 X 104 Jm-2. For 670 nm, significant differences in the proliferation were observed between the two concentrations of FBS and between irradiated cultures and controls. Although the results were not significant, 635 nm irradiated cells also proliferated more than non- irradiated ones. This occurred under both conditions of nutrition. It is concluded, that irradiation with 670 nm laser light applied at doses between 0.04 and 4.8 X 104 Jm-2 could significantly increase proliferation of laryngeal cancer cells.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Antonio Luiz Barbosa Pinheiro, Antonio Luiz Barbosa Pinheiro, Silene Carneiro Nascimento, Silene Carneiro Nascimento, Alessandro L. B. Vieira, Alessandro L. B. Vieira, Aluizio Barros Rolim, Aluizio Barros Rolim, Pedro Soriano da Silva, Pedro Soriano da Silva, Aldo Brugnera, Aldo Brugnera, Fatima A. A. Zanin, Fatima A. A. Zanin, } "Effects of LLLT on malignant cells: study in vitro", Proc. SPIE 4249, Lasers in Dentistry VII, (27 April 2001); doi: 10.1117/12.424490; https://doi.org/10.1117/12.424490

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