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23 May 2001 In-vitro monitoring of redox state of cytochrome oxidase in bone by optical coherence quantitation based on low-coherence interferometry
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Proceedings Volume 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V; (2001) https://doi.org/10.1117/12.427880
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
Abstract
We present optical coherence quantitation technique to monitor the redox state of mitochondria enzyme Cytochrome oxidase (CytOx) in bone tissue by the use of optical coherence tomography (OCT) system. Superluminescent diode (SLD) with its peak emission wavelength ((lambda) = 820nm) on the absorption band of oxidized form of CytOx was used in the experiments. The reflectance returning from the liquid phantoms (naphthol green B with intralipid) and bone tissue specimens (periosteum of calvaria from newborn rats) as a function of penetration depth was used to quantify the absorption changes of the sample. Absorption coefficients of naphthol green B were accurately quantified by the linear relationship between attenuation coefficients from the slopes of the reflected signals and naphthol green B concentration. The results show that the attenuation coefficient decreases in periosteums as CytOx is reduced by sodium dithionite, demonstrating the feasibility of this method to quantify the redox state of tissues studied. A 70% +/- 7% (n=4) reduction of attenuation coefficients in periosteums was clearly observed with redox change of CytOx after 5 min reduction. In addition, the results demonstrate that the OCT system is also capable of imaging the calvaria tomographically with a resolution at 9 microns, which could only be previously obtained by the conventional excisional biopsy.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Xiangqun Xu, Ruikang K. Wang, and Alicia J. El Haj "In-vitro monitoring of redox state of cytochrome oxidase in bone by optical coherence quantitation based on low-coherence interferometry", Proc. SPIE 4251, Coherence Domain Optical Methods in Biomedical Science and Clinical Applications V, (23 May 2001); https://doi.org/10.1117/12.427880
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