10 May 2001 Array biosensor for simultaneous detection of multiple analytes
Author Affiliations +
Proceedings Volume 4252, Advances in Fluorescence Sensing Technology V; (2001) https://doi.org/10.1117/12.426740
Event: BiOS 2001 The International Symposium on Biomedical Optics, 2001, San Jose, CA, United States
The array biosensor has been developed for simultaneous analysis of multiple samples for multiple analytes. A patterned array of capture antibodies is immobilized on the surface of a planar waveguide and a sandwich immunoassay conducted using a cocktail of fluorescent tracer antibodies. Upon excitation of the fluorescent label using a 635 nm diode laser, a CCD camera detects the pattern of fluorescent antigen:antibody complexes on the sensor surface. Image analysis software correlates the position of fluorescent signals with the identity of the analyte. The assays are fast, sensitive, and specific.
© (2001) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Frances S. Ligler, Frances S. Ligler, Joel P. Golden, Joel P. Golden, Chris A. Rowe-Taitt, Chris A. Rowe-Taitt, James P. Dodson, James P. Dodson, "Array biosensor for simultaneous detection of multiple analytes", Proc. SPIE 4252, Advances in Fluorescence Sensing Technology V, (10 May 2001); doi: 10.1117/12.426740; https://doi.org/10.1117/12.426740


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